Introduction This book provides a comprehensive introduction to the field of recombinant DNA (rDNA) technology, a cornerstone of modern biotechnology and genetic engineering. Designed for students and professionals, it explores both foundational principles and advanced applications of rDNA, which allows scientists to manipulate genetic material, enabling groundbreaking advancements in medicine, agriculture, and environmental science. Recombinant DNA Technology: Concepts and Applications begins with an overview of essential prerequisites, including the molecular tools and techniques central to DNA manipulation, such as restriction enzymes, ligases, and polymerases. Readers are then guided through gene transfer methods, vector selection, and screening techniques, which are crucial for successful genetic modification. Subsequent chapters delve into genome mapping and the analytical techniques used to study genetic sequences. Methods like Polymerase Chain Reaction (PCR), gel electrophoresis, and microarray analysis are discussed in detail, highlighting their role in DNA analysis and genetic research. The final sections focus on the transformative applications of rDNA technology, such as gene therapy, stem cell research, and advanced sequencing techniques. These applications underscore the power of rDNA to address genetic disorders, develop new medical treatments, and advance personalized medicine. Through this book, readers will gain both a theoretical understanding and practical insights into the methodologies of recombinant DNA technology, equipping them with the knowledge to contribute to this rapidly evolving field.